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mouse anti fas iii  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti fas iii
    Mouse Anti Fas Iii, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 573 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+fas+iii/anti-Fasciclin+III/pm41166424-359-14-19
    Average 97 stars, based on 573 article reviews
    mouse anti fas iii - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    other:

    Article Title: Post-eclosion growth in the Drosophila Ejaculatory Duct is driven by Juvenile Hormone signaling and is essential for male fertility
    Article Snippet: Mouse Anti-Fas III , Developmental Studies Hybridoma Bank , 7G10 , 1:500.


    Incubation:

    Article Title: Autocrine Wingless constricts the Drosophila embryonic gut by Ca +2 -mediated repolarisation of mesoderm cells
    Article Snippet: .. Secondary antibody incubation was done in PBT-BSA at room temperature (RT) in the dark for 2 h. The following antibodies were used: Rat anti-DE-cad (DCAD2, DSHB) 1:100; mouse anti-Fas-III (7G10, DSHB) 1:20; mouse anti-β-PS (CF.6G11, DSHB) 1:20; rabbit antiEb1 (Rogers et al, ) 1:100; goat anti-GFP 1:300 (Abcam), anti-RFP 1:200 (Abcam) chicken anti-β-gal (Abcam) 1:300, mouse antiKDEL (Stressmarq Biosciences) 1:200, Cyanin conjugated secondary antibodies (Jackson Immuno-Research) 1:300 and Alexa conjugated secondary antibodies (Thermofisher) 1:300. .. Confocal images of fixed embryos were obtained either with a Leica TCS-SPE or a Zeiss 780.

    Article Title: Autocrine Wingless constricts the Drosophila embryonic gut by Ca +2 -mediated repolarisation of mesoderm cells.
    Article Snippet: .. Secondary antibody incubation was done in PBT-BSA at room temperature (RT) in the dark for 2 h. The following antibodies were used: Rat anti-DE-cad (DCAD2, DSHB) 1:100; mouse anti-Fas-III (7G10, DSHB) 1:20; mouse anti-β-PS (CF.6G11, DSHB) 1:20; rabbit antiEb1 (Rogers et al, 2002) 1:100; goat anti-GFP 1:300 (Abcam), anti-RFP 1:200 (Abcam) chicken anti-β-gal (Abcam) 1:300, mouse antiKDEL (Stressmarq Biosciences) 1:200, Cyanin conjugated secondary antibodies (Jackson Immuno-Research) 1:300 and Alexa conjugated secondary antibodies (Thermofisher) 1:300. .. Confocal images of fixed embryos were obtained either with a Leica TCS-SPE or a Zeiss 780.

    Staining:

    Article Title: Histone H3.3 K27M and K36M mutations de-repress transposable elements through perturbation of antagonistic chromatin marks
    Article Snippet: .. Araldite blocks were cut in 1 μm sections and stained with Richardson’s stain (1% methylene blue in 1% borax solution and 1% azur II solution) for examination by light microscopy. . Immunofluorescence Eye-antennal imaginal discs of third instar larvae were dissected, fixed in 3.7% formalin and subsequently stained using primary antibodies [rabbit anti-Tri-Methyl-Histone H3 (Lys27, 1:500 or 1:100, C36B11, Cell Signaling, Cambridge, UK), rabbit anti-Di-Methyl-Histone H3 (Lys27 1:200, D18C8, Cell Signaling, Cambridge, UK), rabbit anti-Tri-Methyl-Histone H3 (Lys36, 1:50, #9763, Cell Signaling), rabbit anti-di-Methyl-Histone H3 (Lys36, 1:100 or 1:25, C75H12, Cell Signaling, Cambridge, UK), mouse anti-Antp (1:200, 8C11, DSHB), mouse anti-Abd-B (1:10, 1A2E9, DSHB), mouse anti-Ubx (1:10, FP3.38, DSHB), mouse anti-Fas III (1:20, 7G10, DSHB), mouse anti-wingless (1:30, 4D4, DSHB), rhodamine phalloidin (1:40, Life Technologies), rabbit anti-Hemagglutinin (1:500, ab9110, Abcam), rat anti-Hemagglutinin (1:100, 3F10, Sigma Aldrich, Munich) and guinea pig anti-Krimper (1:2000, gift from Toshie Kai, Osaka, Japan). .. As secondary antibodies [goat Alexa Fluor ® 488 anti-rabbit, goat Alexa Fluor ® 568 anti-rabbit, goat Alexa Fluor ® 488 anti-mouse, goat Alexa Fluor ® 647 anti-rat (1:1000, Thermo Fisher Scientific, Waltham, MA)], goat AlexaFluor ® 488 anti-mouse (1:500, Invitrogen), goat AlexaFluor ® 594 anti-rabbit (1:500, Abcam), goat AlexaFluor ® 635 anti-rabbit (1:500, Life Technologies) were used.

    Light Microscopy:

    Article Title: Histone H3.3 K27M and K36M mutations de-repress transposable elements through perturbation of antagonistic chromatin marks
    Article Snippet: .. Araldite blocks were cut in 1 μm sections and stained with Richardson’s stain (1% methylene blue in 1% borax solution and 1% azur II solution) for examination by light microscopy. . Immunofluorescence Eye-antennal imaginal discs of third instar larvae were dissected, fixed in 3.7% formalin and subsequently stained using primary antibodies [rabbit anti-Tri-Methyl-Histone H3 (Lys27, 1:500 or 1:100, C36B11, Cell Signaling, Cambridge, UK), rabbit anti-Di-Methyl-Histone H3 (Lys27 1:200, D18C8, Cell Signaling, Cambridge, UK), rabbit anti-Tri-Methyl-Histone H3 (Lys36, 1:50, #9763, Cell Signaling), rabbit anti-di-Methyl-Histone H3 (Lys36, 1:100 or 1:25, C75H12, Cell Signaling, Cambridge, UK), mouse anti-Antp (1:200, 8C11, DSHB), mouse anti-Abd-B (1:10, 1A2E9, DSHB), mouse anti-Ubx (1:10, FP3.38, DSHB), mouse anti-Fas III (1:20, 7G10, DSHB), mouse anti-wingless (1:30, 4D4, DSHB), rhodamine phalloidin (1:40, Life Technologies), rabbit anti-Hemagglutinin (1:500, ab9110, Abcam), rat anti-Hemagglutinin (1:100, 3F10, Sigma Aldrich, Munich) and guinea pig anti-Krimper (1:2000, gift from Toshie Kai, Osaka, Japan). .. As secondary antibodies [goat Alexa Fluor ® 488 anti-rabbit, goat Alexa Fluor ® 568 anti-rabbit, goat Alexa Fluor ® 488 anti-mouse, goat Alexa Fluor ® 647 anti-rat (1:1000, Thermo Fisher Scientific, Waltham, MA)], goat AlexaFluor ® 488 anti-mouse (1:500, Invitrogen), goat AlexaFluor ® 594 anti-rabbit (1:500, Abcam), goat AlexaFluor ® 635 anti-rabbit (1:500, Life Technologies) were used.

    Immunofluorescence:

    Article Title: Histone H3.3 K27M and K36M mutations de-repress transposable elements through perturbation of antagonistic chromatin marks
    Article Snippet: .. Araldite blocks were cut in 1 μm sections and stained with Richardson’s stain (1% methylene blue in 1% borax solution and 1% azur II solution) for examination by light microscopy. . Immunofluorescence Eye-antennal imaginal discs of third instar larvae were dissected, fixed in 3.7% formalin and subsequently stained using primary antibodies [rabbit anti-Tri-Methyl-Histone H3 (Lys27, 1:500 or 1:100, C36B11, Cell Signaling, Cambridge, UK), rabbit anti-Di-Methyl-Histone H3 (Lys27 1:200, D18C8, Cell Signaling, Cambridge, UK), rabbit anti-Tri-Methyl-Histone H3 (Lys36, 1:50, #9763, Cell Signaling), rabbit anti-di-Methyl-Histone H3 (Lys36, 1:100 or 1:25, C75H12, Cell Signaling, Cambridge, UK), mouse anti-Antp (1:200, 8C11, DSHB), mouse anti-Abd-B (1:10, 1A2E9, DSHB), mouse anti-Ubx (1:10, FP3.38, DSHB), mouse anti-Fas III (1:20, 7G10, DSHB), mouse anti-wingless (1:30, 4D4, DSHB), rhodamine phalloidin (1:40, Life Technologies), rabbit anti-Hemagglutinin (1:500, ab9110, Abcam), rat anti-Hemagglutinin (1:100, 3F10, Sigma Aldrich, Munich) and guinea pig anti-Krimper (1:2000, gift from Toshie Kai, Osaka, Japan). .. As secondary antibodies [goat Alexa Fluor ® 488 anti-rabbit, goat Alexa Fluor ® 568 anti-rabbit, goat Alexa Fluor ® 488 anti-mouse, goat Alexa Fluor ® 647 anti-rat (1:1000, Thermo Fisher Scientific, Waltham, MA)], goat AlexaFluor ® 488 anti-mouse (1:500, Invitrogen), goat AlexaFluor ® 594 anti-rabbit (1:500, Abcam), goat AlexaFluor ® 635 anti-rabbit (1:500, Life Technologies) were used.



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